Enzyme Engineering for Biomedical and Environmental Application

Doctoral Thesis

Shapla Bhattacharya, Riga Technical University, Latvia
ORCID iDhttps://orcid.org/0000-0002-0060-2816

The Doctoral Thesis focuses on the rational engineering of enzymes for biomedical and environmental applications. Computational design, structural biology, molecular dynamics simulations, and experimental characterisation were integrated to develop improved variants of fructosyl peptide oxidases (FPOXs) and polyethylene terephthalate hydrolases (PETases). Engineered FPOX variants exhibited enhanced thermal stability and broadened substrate accessibility for diabetes diagnostics, while optimised PETases demonstrated improved plastic-degradation efficiency and stability, contributing to sustainable enzymatic recycling technologies.

Additional information

Publication type

Defence date

23.07.2026.

Format

Pages

168

Publication date

Published online

Publication language

,

Publisher

RTU Press

Country of Publication

Latvia

Funding source